Fig. 4.
![Fig. 4. Increasing concentrations of (A77 × 520C9) BsAb directed to FcαRI and HER-2/neu were analyzed in ADCC against SK-BR–3 breast cancer cells. As effector cell source, unfractionated whole blood (A, •) was compared with human plasma (▵), isolated MNC (○), or PMN effector cells (▪) (B) at an E:T ratio of 80 to 1. Significant ADCC (P < .05 indicated by an asterisk [*]) was observed with BsAb concentrations higher than 0.08 μg/mL in whole blood and with PMN, but not with plasma or MNC. Results from three experiments are presented as mean ± SEM of % specific lysis.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/11/10.1182_blood.v90.11.4485/3/bl_0031f4.jpeg?Expires=1724262326&Signature=z6O8Sd9hLyYqlkoQpeEKWG~iQP-qwg3~Dp~mlLJr4ZjTqASDvr9iiga~1Q-m8xmP6x7~qAI7eqSxdHBOJEE~a1xa8LnnsIYRWZZL5-bVySbsV0ObNF3IqaxSJIv6tCQU2XNPxTuPVq5YeO429~eafISNzv4hi39J5Hl-fLcBOO7biOoqVZE0vWpqRnKqqnExqhM-~9qvy4KVt2NXR4MqIw1nG9AXkemivRdtYmWoCoLDHuyJgwadv5CHjw7enU6j0x49-9y9RvTBgjSlkW1FxLPVkmQeSmYsYzI7kTpoAlHiwlOzOnHu0f8X-haCXiRQ9nGLY1OY0S4izhrbgAOeYQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Increasing concentrations of (A77 × 520C9) BsAb directed to FcαRI and HER-2/neu were analyzed in ADCC against SK-BR–3 breast cancer cells. As effector cell source, unfractionated whole blood (A, •) was compared with human plasma (▵), isolated MNC (○), or PMN effector cells (▪) (B) at an E:T ratio of 80 to 1. Significant ADCC (P < .05 indicated by an asterisk [*]) was observed with BsAb concentrations higher than 0.08 μg/mL in whole blood and with PMN, but not with plasma or MNC. Results from three experiments are presented as mean ± SEM of % specific lysis.
