![Inhibitory effect of IL-10 on IL-2– or IL-15–mediated cytokine production. (A) T cells were isolated by rosetting and then stimulated by anti-CD3 + IL-15 (100 ng/mL) in the presence or absence of IL-10 (0.1 to 10 ng/mL). Depicted are mean and SEM of four independent experiments showing inhibition of cytokine-induced IFN-γ or TNF-α production. % Inhibition of cytokine production by IL-10 was calculated by (1 − [cytokine production in the presence of IL-10]/[cytokine production in the absence of IL-10]) × 100%. (B) T cells were isolated by rosetting and then stimulated by anti-CD3 + IL-2 (100 ng/mL) in the presence or absence of IL-10 (0.1 to 10 ng/mL). Depicted are mean and SEM of four independent experiments showing inhibition of cytokine-induced IFN-γ or TNF-α production. % Inhibition of cytokine production by IL-10 was calculated by (1 − [cytokine production in the presence of IL-10]/[cytokine production in the absence of IL-10]) × 100%.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/11/10.1182_blood.v90.11.4513/3/bl_0040f3b.jpeg?Expires=1720796412&Signature=joH-ZOtFZJWYJNmXNOD3C9RobPZqMCXz8miuNBhJSVzW4U1Re3d6ii1c6-Rz6YK-JUQgBJ4sVU9ylAgyxMMo4z2YbTRlyrVTOMRukQiHNFuiw51ezDU8eREqcJm5LgJnd-0KhImTfS5PUfNTXw00Ba~G4cv1wNIdd66CkXXL9fJMYO9sdqne1KEw6HUGrnaNE~VaaX5ZYXvCS3TfASNMEn7MS2VcuOJCED1QMuJBTcQn8Jhfe9U5~I7YRWqJXZEKyJjaZxG1zDR~BIQCiiWjk1nRdwipSH8AByHReHPJteHfiBbzASml2qOce0JkObKQD2rsutQHiyBtZ1HoD7Cgsw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibitory effect of IL-10 on IL-2– or IL-15–mediated cytokine production. (A) T cells were isolated by rosetting and then stimulated by anti-CD3 + IL-15 (100 ng/mL) in the presence or absence of IL-10 (0.1 to 10 ng/mL). Depicted are mean and SEM of four independent experiments showing inhibition of cytokine-induced IFN-γ or TNF-α production. % Inhibition of cytokine production by IL-10 was calculated by (1 − [cytokine production in the presence of IL-10]/[cytokine production in the absence of IL-10]) × 100%. (B) T cells were isolated by rosetting and then stimulated by anti-CD3 + IL-2 (100 ng/mL) in the presence or absence of IL-10 (0.1 to 10 ng/mL). Depicted are mean and SEM of four independent experiments showing inhibition of cytokine-induced IFN-γ or TNF-α production. % Inhibition of cytokine production by IL-10 was calculated by (1 − [cytokine production in the presence of IL-10]/[cytokine production in the absence of IL-10]) × 100%.
