Unlike the EpoR, GMR or IL-5R do not signal differentiation in Ba/F3 cells. Ba/F3 cells WT and Ba/F3 cells transfected with the indicated receptor chains EpoR, GMR-α, and IL-5Rα were grown in IL-3 (0.5 ng/mL) and G418, or in Epo (0.5 U/mL), GM-CSF (1 ng/mL), and IL-5 (1 ng/mL), as shown above each lane. Total RNA was isolated after 10 days, and probed for β-globin expression. Fifteen micrograms was analyzed in each lane. Loading efficiency was controlled by hybridization of the same blot with a GAPDH probe (shown below).