Fluorescent staining of CD24-coated beads with selectin-IgGs. (A) CD24-coated polysterene beads derived from KS tumor cells were stained with human or mouse P-selectin–IgG chimeric proteins and FITC-conjugated goat antibodies directed against the Fc part of human IgG. (B) Staining of CD24 beads (KS cells) with human P-selectin–IgG in the presence of 2 mmol/L EDTA, an MoAb to P-selectin or soluble CD24. Arrowheads indicate the position of the positive control (see A). (C) CD24-coated latex beads derived from SW2 tumor cells or neutrophils, control beads coated with lens culinaris glycoproteins from SW2 cells, or MHC class I–coated beads were stained with human P-selectin–IgG as described.