Fig. 2.
Fig. 2. Flow cytometric analysis of CXCR-1 and CXCR-2 expression on neutrophils. Cells were incubated with α-CXCR–1 antibody SE-2 (A) or with α-CXCR–2 antibody RII115 (B). Incubation with either antibody was performed in the absence (1) or presence (2) of a 100-fold molar excess of the peptide fragments CXCR-1[1-30] (A) or CXCR-2[6-29] (B). Subsequently, cell-bound antibodies were detected by fluorescein-conjugated goat α-mouse IgG. Control cells received the secondary antibody only (3).

Flow cytometric analysis of CXCR-1 and CXCR-2 expression on neutrophils. Cells were incubated with α-CXCR–1 antibody SE-2 (A) or with α-CXCR–2 antibody RII115 (B). Incubation with either antibody was performed in the absence (1) or presence (2) of a 100-fold molar excess of the peptide fragments CXCR-1[1-30] (A) or CXCR-2[6-29] (B). Subsequently, cell-bound antibodies were detected by fluorescein-conjugated goat α-mouse IgG. Control cells received the secondary antibody only (3).

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