Additive effect of dilazep and adenosine on the TF activity of HUVECs stimulated with TNF, thrombin, or PMA. HUVECs (2 × 105 cells/well) were incubated with (1 μg /mL, final concentration) or without adenosine for 15 minutes and then with adenosine (2.5 μmol/L). After incubation of the cells with 1,000 U/mL TNF, 25 nmol/L thrombin, or 5 nmol/L PMA, in the presence or absence of dilazep or adenosine, the cells were washed twice with 1 mL of HEPES-buffered saline containing 5 mmol/L CaCl2 . TF activity was determined as factor Xa generation on the surface of HUVECs as described in the Materials and Methods. The data are expressed as the percentage of the control (each stimulant induced TF activity in the absence of both dilazep and adenosine). Statistical analysis was performed using the Student's t-test (*P < .05; **P < .005; ***P < .001).
