Effect of 8-SPT on the dilazep-induced downregulation of TF activity on the surface of HUVECs stimulated with TNF, thrombin, or PMA. HUVECs (2 × 105 cells/well) were incubated with (1 mmol/L, final concentration; ▪) or without (□) 8-SPT for 15 minutes and then with 100 μg/mL dilazep. After incubation of the cells with 1,000 U/mL TNF, 25 nmol/L thrombin, or 5 nmol/L PMA, in the presence or absence of dilazep, the cells were washed twice with 1 mL of HEPES-buffered saline containing 5 mmol/L CaCl2 . TF activity was determined as factor Xa generation on the surface of HUVECs as described in the Materials and Methods. The data are expressed as the percentage of the control (each stimulant induced TF activity in the absence of dilazep). Statistical analysis was performed using the Student's t-test (*P < .05; **P < .001).
