Western blot analysis of the effect of dilazep on the molecular mass of TF in HUVECs stimulated with TNF. HUVECs were treated for 5 hours with TNF (1,000 U /mL) in the presence of 100 μg/mL dilazep. The TF antigen in HUVECs were detected by Western blot analysis using polyclonal antihuman TF antibody (see the Materials and Methods).