Effect of dilazep on total TF antigen level in HUVECs stimulated with TNF, thrombin, or PMA. HUVECs were treated for 5 hours with each stimulant: 1,000 U /mL TNF, 25 nmol/L thrombin, and 5 nmol/L PMA, in the presence of varying concentrations of dilazep (0 to 100 μg/mL). The total cell TF antigen levels in HUVECs were measured by ELISA (see the Materials and Methods). The data are expressed as the percentage of the control (TF antigen in HUVECs without stimulation in the absence of dilazep). The values represent the mean ± SD of four independent experiments.