Comparative ability of neutrophils and autologous monocytes to activate NF-κB in response to LPS. Cells (5 × 10⁶/mL) were cultured in the presence or absence ( — ) of 1 μg/mL LPS for 20 minutes at 37°C, and the resulting whole-cell extracts were analyzed in EMSA. The amount of extract used corresponded to 640,000 cell equivalents (neutrophils) or 190,000 cell equivalents (monocytes). This experiment is representative of three. The various DNA-binding complexes are indicated by the letters A, B, and C; pmn, polymorphonuclear neutrophils; mono, monocyte-enriched suspensions.