Transduced dendritic cells express critical costimulatory molecules and surface markers. CD34⁺ bone marrow and cord blood progenitors were cultured in complete IMDM-20% FCS with c-kit-ligand, GM-CSF, and TNFα. Proliferating cells were transduced on day 3 of culture by cocultivation for 24 hours and were recultured in original media with fresh cytokines for an additional 8 to 10 days. Two-color cytofluorographic evaluation was performed with FITC-conjugated rat antimurine CD2 and the indicated PE-conjugated murine antihuman MoAbs. Mock-transduced cells were cocultivated with an empty packaging cell line and are represented here by those derived from cord blood CD34⁺ cells. The percentage of total gated events is indicated in each quadrant.