Adhesion molecules expressed on human mast cells cultured with or without IL-4. Human cultured mast cells (2 × 10⁵) established from CB MNCs were cultured with or without IL-4 (10 ng/mL) for 5 days in the presence of SCF (100 ng/mL) and IL-6 (80 ng/mL), and the expression of various adhesion molecules was analyzed by flow cytometry. Cells were incubated with different murine MoAbs specific for (A) integrins; and (B) adhesion molecules belonging to Ig superfamilies; (━), mast cells cultured with IL-4; —— , cultured without IL-4), or subclass matched control antibodies (- - - -), and were subsequently stained with FITC-conjugated goat antimouse IgG. The results are one representative out of three.