Expression of HTKL in lymphohematopoietic tissues. (A) Northern blots of HTKL expression in lymphohematopoietic tissues. The EcoRI-EcoRI fragment of HTKL cDNA (200 bp) was used as a probe. Loading was normalized using a β-actin probe. (B) RT-PCR analysis of HTKL mRNA. RT-PCR was performed on C-1 (positive control), bone marrow stromal, and bone marrow mononuclear cells with or without reverse transcriptase (RT + or RT −, respectively). Human β-actin was used as a reference gene. The PCR products were resolved by electrophoresis on a 2% agarose gel and stained with ethidium bromide.