Detection of transfected TRA1 mRNA by RT-PCR. Total RNA (0.2 μg) was isolated from TRA1-transfected and mock-transfected Mm-S1 cells and RT-PCR was performed as detailed in the Materials and Methods. In this condition, transfected TRA1 mRNA and GAPDH mRNA (control) were detected as 236-bp and 178-bp bands, respectively. Lane 1, Mm-S1/neo clone 6 cells; lane 2, Mm-S1/TRA1 clone 24 cells; lane 3, Mm-S1/TRA1 clone 24 cells recovered from ascites of Mm-S1/TRA1 clone 24-injected mouse.