Fig. 1.
![Fig. 1. Differential mRNA display of Mm subline cells. Total RNA isolated from Mm-A, Mm-P, Mm-S1, and Mm-S2 cells was reverse transcribed and amplified by PCR in the presence of [35S]dATP. AP-15 (5′-AGGGCCTGTT-3′ ) was used as an arbitrary primer and T12MT (A) or T12MC (B) was used as an anchored oligo(dT) primer. The PCR fragments were displayed on a 6% DNA sequencing gel and autoradiogrammed as detailed in the Materials and Methods. Fragments 15T01 (A) and 15C01 (B) are indicated by arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2975/4/bl_0008f1.jpeg?Expires=1724271771&Signature=MX4cpV7RftzKOan5UVAgNrn3I8twMfZyTZ1d~ITItM~-r0TiMPtRO1uoZtQLzWa188A4q3Y2wp7yNVOT1aBg1c5~uRevG57n7jT3QyUvT4h2VAnlA9VgCAyOIL7BzdPjH7fMBddKShjhgS23otfAxf9imX~SvyTK5olH4RBUs0MAvXxunF2UMs1OkRUHEJzLZZqc23w9RBJwrf-2OWfid-giX50XmYeuP0r5rC23Q4eSBbicw2TCQ2zM2US07QyVbpMCvI9bZnh2MuYyCHTWSbER9sMuu9F9BkiyWeskT5kqzD6U2tFzwUKjz0bkFBfhWhFQhd373AVmP3Jdndc~gQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Differential mRNA display of Mm subline cells. Total RNA isolated from Mm-A, Mm-P, Mm-S1, and Mm-S2 cells was reverse transcribed and amplified by PCR in the presence of [35S]dATP. AP-15 (5′-AGGGCCTGTT-3′ ) was used as an arbitrary primer and T12MT (A) or T12MC (B) was used as an anchored oligo(dT) primer. The PCR fragments were displayed on a 6% DNA sequencing gel and autoradiogrammed as detailed in the Materials and Methods. Fragments 15T01 (A) and 15C01 (B) are indicated by arrows.
