Fig. 1.
![Fig. 1. Autoradiograph of various proteolytic fragments of CDB3 phosphorylated by casein kinase I and [δ-32P]ATP. CDB3 was phosphorylated by casein kinase I using radiolabeled ATP, as described in the Materials and Methods. Phosphorylated CDB3 was then either left unmodified or digested at specific sites and separated by Tricine SDS-PAGE. Lane A, intact CDB3; lane B, proteolytic fragments generated by NTCB cleavage; lane C, fragments produced by NH2OH hydrolysis; and lane D, fragments released upon digestion in formic acid. Lane E shows the position of molecular weight markers.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.3019/4/bl_0040f1.jpeg?Expires=1724263123&Signature=ihOQx6Ik4h9rsXYDaw75QfymRZksgKj~Zd8o1NJzMvMEq3mMrf3U00wNAqHxQk1OBUwXCMDESfmReYixGIYC~Bi-LOIjXSMhGr4t6cf~M2gh0GP2ou0rlIK6SavtZz4x~zRdUWfhCCocxMTowzlyIFWPBagNvPamN-u2C4Ystdnu4sJmXiw3sD1a~AgPJuQm~b6fCSpmL8zIlUEoWLcGygL1yN2yuFhgegaDkyA3IpdT9auRqXVKPgcJ7PXtmm75~zLuc-oEhclTbvHBoBiyN010963Wyc9CgtIJ2tvmFD5teJ0snHjfBJjOJ7ndTsl7MNOw2kIYvTYoOF0BQUL-6Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Autoradiograph of various proteolytic fragments of CDB3 phosphorylated by casein kinase I and [δ-32P]ATP. CDB3 was phosphorylated by casein kinase I using radiolabeled ATP, as described in the Materials and Methods. Phosphorylated CDB3 was then either left unmodified or digested at specific sites and separated by Tricine SDS-PAGE. Lane A, intact CDB3; lane B, proteolytic fragments generated by NTCB cleavage; lane C, fragments produced by NH2OH hydrolysis; and lane D, fragments released upon digestion in formic acid. Lane E shows the position of molecular weight markers.
