Fig. 6.
![Fig. 6. Effect of Z-VAD.fmk on the viability of B-CLL cells. Cells from five patients were seeded in 96-microwell plates and incubated for 48 hours with the indicated factors. The concentrations used were 10 μmol/L dexamethasone and 200 μmol/L Z-VAD.fmk. Z-VAD.fmk was added 1 hour before dexamethasone administration. Cytotoxicity was analyzed by the MTT method as described in Materials and Methods. Data are shown as the mean value ± SD of two (patients 6, 8, and 9) or four (patients 5 and 7) independent experiments. Statistical significance of differences between treatments without or with Z-VAD.fmk was assayed by analysis of variance (ANOVA) (Fisher's Protected Least Significant Difference [PLSD]): * = P < .12, ** = P < .05, *** = P < .01.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/9/10.1182_blood.v89.9.3378/3/bl_0038f6.jpeg?Expires=1725125139&Signature=UR~0FXmaBVmRIpag8BqtNvvSSwmcu5lyHdhwzISfnpx70AxQ5Hj0LqyMMDB35gUQQjK3mHWwM3agVq7baQFfEAQDXQ~iaHzr6scAX-2LJVhy3VYUqXgUlbUHLSax4VxEkeFYZPvwlsoFDStJfjMmj50IntAVMVBEqSPmn7Z3tka11eKlG7CekI6D1DLRoUzVOpo~fntuT7nQD4Dn6jeS01APmFyPdUt3VsdEPFJ7X2LM1-bGdYiIO8A~lG0gBNc~ZNBQ7XFtkKS-DO49eyPdYhxKqKC3kL9JVTrpE6T04goEHDVDPKRDLif4aITC1AQZQlCAKR9hCTygmDmKH~Taow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Z-VAD.fmk on the viability of B-CLL cells. Cells from five patients were seeded in 96-microwell plates and incubated for 48 hours with the indicated factors. The concentrations used were 10 μmol/L dexamethasone and 200 μmol/L Z-VAD.fmk. Z-VAD.fmk was added 1 hour before dexamethasone administration. Cytotoxicity was analyzed by the MTT method as described in Materials and Methods. Data are shown as the mean value ± SD of two (patients 6, 8, and 9) or four (patients 5 and 7) independent experiments. Statistical significance of differences between treatments without or with Z-VAD.fmk was assayed by analysis of variance (ANOVA) (Fisher's Protected Least Significant Difference [PLSD]): * = P < .12, ** = P < .05, *** = P < .01.
