Cyclin D1 expression in peripheral blood, bone marrow, and lymph nodes. (A) Peripheral blood (100 μL each, lanes 2-22) of patients in the hematology outpatient clinic were subjected to RNA extraction and the competitive RT-PCR as described in Materials and Methods. (B) Lymph node cells of a patient with necrotizing lymphadenitis (lane 25) and of lymphoma patients (lanes 26-31) and bone marrow aspirates (50 μL each, lanes 32-35) of lymphoma patients without bone marrow involvement were also subjected to RNA extraction and the competitive RT-PCR. Diseases affecting these patients were as follows: lanes 2, 11, and 22, pernicious anemia; lanes 3 and 5, collagen disease; lane 4, idiopathic thrombocytopenic purpura; lane 6, lymphoplasmacytic lymphoma with bcl-1 rearrangement and cyclin D1 overexpression; lane 7, acute myeloid leukemia in complete remission; lanes 8 and 26-28, follicular lymphoma; lane 9, pulmonary infiltrate with eosinophilia; lanes 10 and 29-31, diffuse lymphoma; lanes 12, 13, 18, and 19, non-Hodgkin's lymphoma in complete remission; lanes 14 and 20, chronic myeloid leukemia in the accelerated phase; lane 15, aplastic anemia; lane 16, myelodysplastic syndrome; lane 17, autoimmune hemolytic anemia; lane 21, iron-deficiency anemia. Negative controls of PCR without added template are in lanes 23 and 36. The sizes (bp) of 100 bp DNA ladders (lanes 1 and 24) are shown on the left.