Comparison between competitive RT-PCR and Northern analysis. RNAs extracted from the indicated cells on each lane were subjected to the competitive RT-PCR (upper part) and to Northern analysis (middle part). Cell lines are on lanes 2 through 10, 17, 18, and 19. Mononuclear cells from patients with multiple myeloma (MM),38 mantle cell lymphoma (MCL), B-cell acute lymphoblastic leukemia (ALL), or B-cell prolymphocytic leukemia (PLL) are on lanes 11 through 16. Mononuclear cells were obtained from bone marrow (lanes 11-13) or peripheral blood (lanes 14-16). Asterisks (*) denote that t(11; 14)(q13; q32) were documented. RNA extraction, the competitive RT-PCR, and Northern analysis were performed as described in Materials and Methods. Negative control of PCR product in the absence of any template is in lane 20. Arrows indicate PCR products corresponding to cyclins D1, D2, and D3 in the upper part, and cyclin D1, D2, and D3 transcript signals in the middle part. A dashed arrow indicates an aberrant cyclin D1 transcript observed in SP-49 cells.39 The sizes of 100-bp DNA ladders (lane 1) and ribosomal RNAs are shown on the left. RNA loading in each lane is shown in the photograph of the ethidium bromide-stained gel at the bottom.