Fig. 3.
Competitive PCR amplified each D-type cyclin sequence specifically and each PCR product served as a competitor. (A) Cyclin D1, D2, and D3 cDNA plasmids (+, 1 × 106 molecules per reaction; −, none) were subjected to the competitive PCR. The sizes of 100 bp DNA ladders (lane 1) are shown on the left. (B) The amount of the indicated plasmid was changed as indicated ratios over the other two and the competitive PCR was performed. Arrows indicate PCR products corresponding to cyclins D1, D2, and D3.
