Fig. 2.
![Fig. 2. Kinetics of the competitive RT-PCR. RNAs extracted from MEG01s and SP-49 cells were subjected to competitive RT-PCR in the presence of [α-32P]dCTP. PCR products were harvested after the indicated cycle of the PCR, separated on a polyacrylamide gel, and visualized by autoradiography (upper part). Arrows indicate the specific PCR products corresponding to cyclins D1, D2, and D3. Gel pieces corresponding to the PCR products of cyclins D1 (•), D2 (○), and D3 (▵), were excised and the radioactivity was counted. [α-32P]dCTP incorporation was plotted on a logarithmic scale over number of cycles of PCR (lower part).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/3/10.1182_blood.v89.3.965/8/bl_0001f2.jpeg?Expires=1722820607&Signature=lMHRcd8Zh2p7YlZcn8GygtfOJXU8UJwO57~fgbpfx-IuOoQhzEwTifVOZBlxsFljBcofCULQrBJpZSdZgYF0tk56tAziNgfEVQvJBijy9ofvxK~vhwWKNVe0h0dLQuqJmdn86ujhY1f1yaBDZTCLWibFQzK1SiO2qD5pfAxxKW~16-KqRVuTqixqcddgEwvOTHI4WbCcr0LcngLF3oL4bc01BLbpUn7o0KIFtOjclHqGDRZoQpsnIoLBytEYaXR2Sz4O8wFoH8j8UqoxCDJb~V-pCPf88ZsL6QRHOLnp9a5x0-fKori1LEr0-Eb1JixQt8233yk18RRibUT4XdyXgQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Kinetics of the competitive RT-PCR. RNAs extracted from MEG01s and SP-49 cells were subjected to competitive RT-PCR in the presence of [α-32P]dCTP. PCR products were harvested after the indicated cycle of the PCR, separated on a polyacrylamide gel, and visualized by autoradiography (upper part). Arrows indicate the specific PCR products corresponding to cyclins D1, D2, and D3. Gel pieces corresponding to the PCR products of cyclins D1 (•), D2 (○), and D3 (▵), were excised and the radioactivity was counted. [α-32P]dCTP incorporation was plotted on a logarithmic scale over number of cycles of PCR (lower part).
