Fig. 2.
![Fig. 2. IL-3, Epo, and Tpo specifically activate both JNK1 and JNK2. FDC-P2 cells (left 2 lanes), FD-EPO cells (middle 2 lanes), and FD-TPO cells (right 2 lanes) were stimulated with IL-3, Epo, or Tpo, respectively, for 0 minutes (−) or 15 minutes (+). In-gel JNK assays were performed in the immunoprecipitates with anti-JNK1 (A) or anti-JNK2 (B) antibody. The immunoprecipitates were separated by SDS-polyacrylamide gels containing GST-c-Jun and incubated with [γ-32P]ATP after protein renaturation. The phosphorylated GST-c-Jun corresponding to the molecular weights of JNK1 (46 kD) and JNK2 (55 kD) are indicated by arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2664/4/bl_0055f2.jpeg?Expires=1724158608&Signature=szNx-TZf6dqnr1VDNmHg6K8tQ9-xwhTaUHHhnpGKgaQMnDP0lYzPsCSiz6jjE4F8i2fspBt9rRgOH7zzh2m9wRdIShOZ4jDjoCHnDRzfRQurOMSq7O4p-guzXzpwxssGiCMYodAayC3MwB3St~~5EM0nDl9m~~UpcUTmM3a7iUHDJpowNOHTRGHF5B8j0K2NSp~xbvZVsZj6iDgt69XRzzm8fXLsRbU-~HzFQ2Yw8KDq4qAgS6Fh-SRibm8-6F-oR6FUQoGwylvZR~hTJItOf43~hwxeGdquCSc1QIF6gdhwERomLwU1kmwDDya3pHvcylrG41WvzAy~MpMvzrTZCg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-3, Epo, and Tpo specifically activate both JNK1 and JNK2. FDC-P2 cells (left 2 lanes), FD-EPO cells (middle 2 lanes), and FD-TPO cells (right 2 lanes) were stimulated with IL-3, Epo, or Tpo, respectively, for 0 minutes (−) or 15 minutes (+). In-gel JNK assays were performed in the immunoprecipitates with anti-JNK1 (A) or anti-JNK2 (B) antibody. The immunoprecipitates were separated by SDS-polyacrylamide gels containing GST-c-Jun and incubated with [γ-32P]ATP after protein renaturation. The phosphorylated GST-c-Jun corresponding to the molecular weights of JNK1 (46 kD) and JNK2 (55 kD) are indicated by arrows.
