Fig. 1.
![Fig. 1. IL-3, Epo, and Tpo activate both JNK1 and JNK2 in in vitro kinase assay. FDC-P2 cells (upper panel), FD-EPO cells (middle panel), and FD-TPO cells (lower panel) were stimulated with IL-3, Epo, and Tpo, respectively, for the indicated time up to 120 minutes. JNK1 (A) and JNK2 (B) activity was measured in the immunoprecipitates with anti-JNK1– and anti-JNK2–specific antibody and [γ-32P]ATP and GST-c-Jun as a substrate. Arrows indicate the phosphorylated GST-c-Jun (molecular weight, 35 kD).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2664/4/bl_0055f1.jpeg?Expires=1724158325&Signature=HmLH6lNeieosXF9~n9lXL5pUF0BfUCSqVY-nwj8mAmRVYJwAeGOyG0QhPQD7IGrGdyZ9oteGTjkYe6oT7tQ3ElBxRr6RSA5dq5M6LS2F~Yi8NbhF75~OAwvWALm9e5ABMxisgaWMlbrFC9W9UWeOaOKqnr-K0gy1hkUn~JQ0MxxhEWusx82Mq69WSdlF2XPVjYtneoYRMWvxWVRtc869w6RUdJN5OTkYffcG6~tZZNdpb4W8Zu7sQeelSUfMyPGShYBBS4tjglkHSvFg3lalkdVmaj67GG-IXyPWyQhHJbdykh8Nb0WqH3VsJob-2nDUbNoccPG0t0MzdLYstbhTcw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-3, Epo, and Tpo activate both JNK1 and JNK2 in in vitro kinase assay. FDC-P2 cells (upper panel), FD-EPO cells (middle panel), and FD-TPO cells (lower panel) were stimulated with IL-3, Epo, and Tpo, respectively, for the indicated time up to 120 minutes. JNK1 (A) and JNK2 (B) activity was measured in the immunoprecipitates with anti-JNK1– and anti-JNK2–specific antibody and [γ-32P]ATP and GST-c-Jun as a substrate. Arrows indicate the phosphorylated GST-c-Jun (molecular weight, 35 kD).
