PCR-SSP analysis of 1 SH(+) (lane 2) and 3 SH(−) phenotyped individuals (lanes 3 to 5) using SH(+)-specific primer no. 7 (A) and SH(−)-specific primer no. 8 (B). The 191-bp bands represent the allele-specific PCR products and the HGH bands the internal controls. PCR products were analyzed on 1.5% agarose gel stained with ethidium bromide. DNA size standards (pBr 328 DNA.BglI + pBr 328 DNA.HinfI) are shown in lane 1. Only in donor 1 was a SH(+)-specific PCR product (A, lane 2) formed.