Comparison of the proportion of CD4⁺Fas^hi and CD8⁺Fas^hi cells in control and MG PBL. PBL freshly isolated over Ficoll gradients were stained with anti-Fas, anti-CD4, and anti-CD8 antibodies. The percentage of CD4⁺ and CD8⁺ peripheral lymphocytes and the proportion of Fas^hi cells among these subsets was determined. Two groups of patients (anti-AChR antibody titer <1 nmol/L, ≥1 nmol/L) are distinguished and compared to controls by using the Mann-Whitney test. No significant modification in the proportion of Fas^hi was observed in MG patient peripheral T-cell subsets.