Fig. 7.
![Fig. 7. A competitive immunoassay showing the effect of prelabeling TSP with NEM on conversion to the EDTA-conformation. Supernatant TSP was derivatized with [3H]NEM in the presence of 2 mmol/L Ca2+. The labeled TSP was purified by heparin-affinity chromatography as described in Materials and Methods. Three hundred fifty micrograms/milliliter Ca-TSP or NEM-TSP was incubated at 37°C for 30 minutes before addition of MoAb D4.6 as described in Fig 5. The results are the means ± SE of 3 separate experiments. (□), 2 mmol/L Ca2+; (○), 6 mmol/L EDTA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/9/10.1182_blood.v89.9.3205/3/bl_0049f7.jpeg?Expires=1723263313&Signature=qNVjkoS7~dlMCUW5s38V8dmuSusskPTGa~Y5x8H8QcggLdPY7XBUc5m8g-yFxNuoHKOe5YETCAUUNTzL1EREo9JsC261w5-uRec3K73m61sSzB3oKmfbhl3aPwczE7R3cIpNEX3SPNqbv6j58Z5TdlcjwlUHK2ISV3CW2K3DBctx3e1tWrqBBMlXu60Mf4J1w8rSbiuqfAoanGBA22v~Yphqo1DeBrmbL1mTpLlgd3SjchDdeej8lZw92Lt9coFNGwcC-1yoWAT33v9CooNXrqb3FqKrtR0TB~Oe6d8xXsFUqsdXY4jSV8kdIVsj9ZRx9Eum7qtV1XIa0TaGEYcy~g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
A competitive immunoassay showing the effect of prelabeling TSP with NEM on conversion to the EDTA-conformation. Supernatant TSP was derivatized with [3H]NEM in the presence of 2 mmol/L Ca2+. The labeled TSP was purified by heparin-affinity chromatography as described in Materials and Methods. Three hundred fifty micrograms/milliliter Ca-TSP or NEM-TSP was incubated at 37°C for 30 minutes before addition of MoAb D4.6 as described in Fig 5. The results are the means ± SE of 3 separate experiments. (□), 2 mmol/L Ca2+; (○), 6 mmol/L EDTA.
