![Fig. 3. Allogeneic responses of lymphocytes from rD-mPGPtide–treated chimeric mice in MLR. Recipient (B6CB)F1 mice were irradiated (750 cGy) and received transplants of MHC-haploidentical (B6D2)F1 ATBM (107 cells). One hour later, mice were injected with PBS (IV), anti-CD4 MoAb (ascites fluid at 25 μg; IP), or rD-mPGPtide (0.5 mg; IV) at 0.2 mL each. On day 70 post-BMT, LN cells from three mice of each treatment group were isolated and used in a MLR as responder cells (2.5 × 105 cells/well). Irradiated (20 Gy) allogeneic DBA/2 (H2d) and SJL (H2s) spleen cells (5 × 105 cells/well) were used as stimulators. Background counts were determined using irradiated syngeneic stimulator cells (5 × 105 cells/well). Proliferative responses of cells from mice in each of the three experimental groups were measured on days 3 through 5 by [3H]TdR incorporation and the SI was calculated relative to the syngeneic background response. The data shown are from one representative experiment of two performed for day-4 proliferative responses. Values are the mean ±SD of quadruplicate cultures.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2880/4/bl_0031f3.jpeg?Expires=1725467414&Signature=RItyniE8le1WzUbY3lR1JPhZJtOUYSinA8FJ4qSeu83vpph~8kVWVigd0MX5NyCila~lyZO8V3jpd1rm2wgs0pGjC535qEFTL51-C~MbIFoQOQ3i7bKSKu6b0FPmWBoxJXrzk0zk7-fBUpSQAEmoXyOIKnGLliqB5covoqgHl69tafzLJA33VxBROzf8rlte9qaNz2WsegzLBmEyoJCNmsorpvf4NaRJ6-4qPpAqLR17t8p2rWznAWttaoNgr5F0uxe3W9sE5TO-vo8HxXus~6s6rCa0rzirIarC8AzJ0tcn1qSpbrx-2fp5LiW5nlxKgFM7HCeHdAmXRLgOMFMNqg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Allogeneic responses of lymphocytes from rD-mPGPtide–treated chimeric mice in MLR. Recipient (B6CB)F1 mice were irradiated (750 cGy) and received transplants of MHC-haploidentical (B6D2)F1 ATBM (107 cells). One hour later, mice were injected with PBS (IV), anti-CD4 MoAb (ascites fluid at 25 μg; IP), or rD-mPGPtide (0.5 mg; IV) at 0.2 mL each. On day 70 post-BMT, LN cells from three mice of each treatment group were isolated and used in a MLR as responder cells (2.5 × 105 cells/well). Irradiated (20 Gy) allogeneic DBA/2 (H2d) and SJL (H2s) spleen cells (5 × 105 cells/well) were used as stimulators. Background counts were determined using irradiated syngeneic stimulator cells (5 × 105 cells/well). Proliferative responses of cells from mice in each of the three experimental groups were measured on days 3 through 5 by [3H]TdR incorporation and the SI was calculated relative to the syngeneic background response. The data shown are from one representative experiment of two performed for day-4 proliferative responses. Values are the mean ±SD of quadruplicate cultures.
