Lymphocyte function in mutant mice. (A) Proliferation assay. T-cell enriched splenocytes and lymph node cell suspensions were prepared and stimulated with Con A (2.5 μg/mL), soluble hamster antimouse CD3 (10 μg/mL), or PMA (125 μg/mL) + Ca Ionophore (50 μL/mL). At indicated time points, proliferating cells were pulsed with 1 μCi 3H-thymidine and cultured overnight. Data represent average 3H-uptake of quadruplicates (closed triangles, +/−; open triangles, −/−). Similar results were obtained in three independent experiments. (B) MLRs were performed by mixing irradiated stimulator spleen cells (1 × 105) from C57BL/6, BALB/c, B10.BR, BM1, or BM12 mice with responder T-cell enriched splenocytes and lymph node cells (1 × 105) harvested from +/− (closed triangles) or −/− (open triangles) mice. Each line represents an individual mouse. (Results are shown for days 3, 4, and 5; similar results were obtained in three independent tests when littermates were used as responders.)
