Effect of HIV recombinant gp120 on IL-10 mRNA accumulation in monocytes. Elutriated monocytes were cultured with HIV recombinant gp120. Cells were harvested for IL-10, p35, p40, and GAPDH mRNA assay at 4 hours or 24 hours after the beginning of the culture. For each gene, the 0.1-fg-point of purified PCR product amplified in the same experiment is shown. Each point plotted represents the mean of duplicate culture samples. Similar results were obtained in another experiment.