Effect of HIV recombinant gp120 on IL-12 p35 and p40 mRNA accumulation in monocytes and macrophages. (A) Monocyte-enriched adherent cells were preincubated or not with HIV recombinant gp120 for 24 hours and then activated with SAC for 4 hours. Cells were harvested for mRNA assay. Each value represents the mean ± SEM of quadruplicate culture samples. Similar results were obtained in two other experiments. (B) Seven-day macrophages (obtained as described in the Materials and Methods) pretreated or not with HIV recombinant gp120 for 24 hours were activated by SAC for 4 hours. Each value represents the mean ± SEM of duplicate culture samples.