Fig. 3.
Fig. 3. Chemiluminescence assay for superoxide production by PMA activated neutrophils derived from sham treated and transduced CD34+ cells at 17 days of culture. The vertical axis represents the average light unit integral over 40 minutes as a measure of superoxide production. Bar A is the average of six cultures of sham treated normal CD34+ cells. Bars B through F represent the average value of four experiments each with p67phox deficient CGD CD34+ cell cultures for each bar seen. Bar B is sham treated patient cells; bars C through F are patient cells transduced with MFGS-p67phox derived from clones 2, 5, 20, 37, respectively. Error bars represent standard error of the mean.

Chemiluminescence assay for superoxide production by PMA activated neutrophils derived from sham treated and transduced CD34+ cells at 17 days of culture. The vertical axis represents the average light unit integral over 40 minutes as a measure of superoxide production. Bar A is the average of six cultures of sham treated normal CD34+ cells. Bars B through F represent the average value of four experiments each with p67phox deficient CGD CD34+ cell cultures for each bar seen. Bar B is sham treated patient cells; bars C through F are patient cells transduced with MFGS-p67phox derived from clones 2, 5, 20, 37, respectively. Error bars represent standard error of the mean.

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