Fig. 7.
![Fig. 7. Association of mi with USF2 in activated MC-9 cells. [35S]-methionine–labeled activated cells (IL-3, IgE-DNP, or IL-4) were lysed with RIPA and incubated with protein A-bound anti-mi antibody. Immunocomplexes were then dissociated from the beads and either reprecipitated or not on protein A bound USF2 (A). Alternatively, [35S]-methionine–labeled IL-3–activated MC-9 cells were lysed with RIPA and incubated with protein A-bound anti-USF2 antibody. The immunocomplexes were then dissociated from the beads, diluted, and reprecipitated on either protein A-bound mi (B) or myosin (C) antibodies. Analysis was by SDS-PAGE. One representative experiment of three performed is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2999/4/bl_0007f7b.jpeg?Expires=1724275436&Signature=4ckeIj6oRM2AP0~0vZPaQ2kckhdYLavy-HR4Vp86RBKUNyIbeO64~HbiTu17TVMupQAxR-3BHcMbsFKsCQdnMFK1ZoWOE~iw6C8fRJi~8BF6k10mzVGIqOVrQ5OLW6av4SaVz59UEvRUvCt4pIT8ysVTIHFfcYMGOy03o~dVf2-fHBpl7sLlv7PGupft6sWTg16qQLOMRcHiVsICy4jjeCtGeKRoS8lBrQT88nLTqGXPuhQImifbimIjqJTEdr4clH0YaC6doBkLbiQIy4fy04ErzDxakQ8LbpvDxQWixlJIXqwZOKUo3Bue~XCI0Xglr1jXOg5P033CKYHC8SKfqg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Association of mi with USF2 in activated MC-9 cells. [35S]-methionine–labeled activated cells (IL-3, IgE-DNP, or IL-4) were lysed with RIPA and incubated with protein A-bound anti-mi antibody. Immunocomplexes were then dissociated from the beads and either reprecipitated or not on protein A bound USF2 (A). Alternatively, [35S]-methionine–labeled IL-3–activated MC-9 cells were lysed with RIPA and incubated with protein A-bound anti-USF2 antibody. The immunocomplexes were then dissociated from the beads, diluted, and reprecipitated on either protein A-bound mi (B) or myosin (C) antibodies. Analysis was by SDS-PAGE. One representative experiment of three performed is shown.
