Fig. 6.
![Fig. 6. Pulse chase experiment. MC-9 cells were incubated for 30 minutes at 37°C in DMEM methionine-free medium containing 50 μCi/mL [35S]-methionine, washed with RPMI 1640 medium, and incubated for various periods of time in RPMI 1640 medium. Zero minute represents the time when the incubation was started after the pulse chase was performed. The [35S]-mi was immunoprecipitated as described in Fig 4. One representative experiment of three performed is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2999/4/bl_0007f6.jpeg?Expires=1724275939&Signature=Tg9yRPJVWUhMAMKG2WmbMiY3W0LxEniOx9PbgeRBINTTFWpMuHliewyvsBy~x6Gz6np2LCjFFjmLtNU5sl4zd9353XI9IcFUOgk~Vgi63JBov9q7VPY4JlYNHd1L~nvY-O7f6jNGYE2cwmXM1C6W687BgweeS5ceMtTNP-aqlVjjoEFbvIbInrVb4IatYlaMbw9SqxlmgiY0IV4Kcn6zxBvJUjSssl8~5CVrjaEJkNwz2gvreVB4GIzEwHbzq8GY0Hnk-GcDaWK~qGzSHz6Lj8tXimwIlAXi8SFJHzIH5AIyo2SwkEe5kB6eNbzYiMXtCVzR6cgEXVo~iH~F2cQyBQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse chase experiment. MC-9 cells were incubated for 30 minutes at 37°C in DMEM methionine-free medium containing 50 μCi/mL [35S]-methionine, washed with RPMI 1640 medium, and incubated for various periods of time in RPMI 1640 medium. Zero minute represents the time when the incubation was started after the pulse chase was performed. The [35S]-mi was immunoprecipitated as described in Fig 4. One representative experiment of three performed is shown.
