Fig. 4.
![Fig. 4. Identification of mi protein in mast cells. MC-9 cells were labeled with [35S]-methionine. mi was identified by preincubating the antibody with or without the mi peptide before the addition of the antibody to the cell lysates (A). Immunoprecipitates of [35S]-methionine–labeled mi from either MC-9, spleen, or liver cells are shown in (B). Immunoprecipitates of [35S]-methionine–labeled mi from either BMMC or BM cells are shown in (C). Immunoprecipitates were analyzed by SDS-PAGE. One representative experiment of two performed is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2999/4/bl_0007f4b.jpeg?Expires=1724275159&Signature=4ixxd6tP15KH1qR~Uoj7fPQY0qtJe0yO8ydQTTHSvtZlWG-DFL0fP1J1cu2PUV9ioQ4-olInu9nGLvBPkYZIWnHHX1ON3D1P4H2HxHh57NOoUyHFwrlkixwE1gPSIHgxqEphSCO-v2YBwZ4gC8R8-VeS7X2aNNfE9rWtTqdIaJE6R8tsqXkf2DZVMQrhBvFkiGc9YrDa0taLYOOIAqd1r510iAnRDTOEqFnhRWsj53bSvnp8D6aYtBNkPzD1KiEQxZVDDx-haZKlZ~xLBcVDYpG0FtGQKsH9KtC-31gbmZQM0DNV6lGXj6v-E7AI3JKLe-CIaqqTmJwkwPSMwUFn2Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Identification of mi protein in mast cells. MC-9 cells were labeled with [35S]-methionine. mi was identified by preincubating the antibody with or without the mi peptide before the addition of the antibody to the cell lysates (A). Immunoprecipitates of [35S]-methionine–labeled mi from either MC-9, spleen, or liver cells are shown in (B). Immunoprecipitates of [35S]-methionine–labeled mi from either BMMC or BM cells are shown in (C). Immunoprecipitates were analyzed by SDS-PAGE. One representative experiment of two performed is shown.
