Fig. 2.
![Fig. 2. Activation of HPg by SK. wtr-E1-HPg (10 nmol/L) was activated by SK (10 nmol/L) in the presence of the chromogenic substrate, S2251 (0.9 mmol/L). The HPm generated as a function of time was detected by the liberation of p-nitroanilide from the substrate and is depicted here as the absorbance at 405 nm. The curves illustrated are for wtr-E1-HPg and for the mutant, r-[R561A]E1-HPg. The reduced DodSO4/PAGE gel insert shows the protein components present after 30 minutes of activation. The buffer was 10 mmol/L HEPES-NaOH, pH 7.4, at 37°C.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/5/10.1182_blood.v89.5.1585/3/bl_0001f2.jpeg?Expires=1724289190&Signature=LYrTLQrIjm2PFd2BzVtGz9VpKxC-CRVkYAz6b0A9dXUE3IajAuTLXPOM0cUyWAvaJ6XprRFtNFimriB9QhOEhfvcuUSNfpAmISJ5PUOST-kjdbO029C64APhDdgEH6Bq-Cg2qEdLrePPwcZWfTZZll8mQQY6imokkonBkD9ykVZx1qoodSkblPQQHSpuvHHfIEIng0wNa9euRDLQ8BnYiH3bZqXBILHZX2AFtUrscsfD5tf9HaO-5nHGVfzWi~lckeX96FvRt0lBHCJO8stdbSrKJKaIz8X~kA2V4kI57PTYQr6YJGBdJ6cTBUI9wltucCpV2ARD9O0rBfI3FSDTGA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of HPg by SK. wtr-E1-HPg (10 nmol/L) was activated by SK (10 nmol/L) in the presence of the chromogenic substrate, S2251 (0.9 mmol/L). The HPm generated as a function of time was detected by the liberation of p-nitroanilide from the substrate and is depicted here as the absorbance at 405 nm. The curves illustrated are for wtr-E1-HPg and for the mutant, r-[R561A]E1-HPg. The reduced DodSO4/PAGE gel insert shows the protein components present after 30 minutes of activation. The buffer was 10 mmol/L HEPES-NaOH, pH 7.4, at 37°C.
