Fig. 1.
![Fig. 1. Cross-linking of [125I]GM-CSF to ECs (A) and U937 cells (B) and displacement by GM-CSF. Quiescent ECs at passage II and U937 cells were incubated in binding medium with 0.5 nmol/L [125I]GM-CSF without or with 100-fold excess of unlabeled ligand in the presence of 1 mmol/L disuccinyl suberate. Proteins were separated by SDS-PAGE and visualized by autoradiography. Three experiments with ECs and 2 with U937 cells have been performed with similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/3/10.1182_blood.v89.3.863/8/bl_0039f1.jpeg?Expires=1722799621&Signature=ImTrFwytVQLo7TLGDD-bE-N2h6yzFIBFFLXINT2aDote2vomwNLQWAnPUDcQahIjtIFTsZpsSrYsFzU57-pmvp9Jdbbmr18PDgbs509fCaASqssTK9eJBC5QaJnJZTGzFqRWIbDgK05t6ZEbgSsCldJkyJlNLYyEG1Ua0ECeqfRqfw9346zEi8SxsNZ9s6cQ-QN2AXd06mRbSAiUvwIl11VrxkVdE~npFq5Xky5weGwfRY6nmUUfZDjDwSOdcADbKQuBGhhTgQNRTEKgh-8Dcl0H5as5so0j17u2nbsXvVnuL42CIinW5efI7qfBmIWZg3IzK7D8WRUJspBwmgoRTQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cross-linking of [125I]GM-CSF to ECs (A) and U937 cells (B) and displacement by GM-CSF. Quiescent ECs at passage II and U937 cells were incubated in binding medium with 0.5 nmol/L [125I]GM-CSF without or with 100-fold excess of unlabeled ligand in the presence of 1 mmol/L disuccinyl suberate. Proteins were separated by SDS-PAGE and visualized by autoradiography. Three experiments with ECs and 2 with U937 cells have been performed with similar results.
