Quantitative analysis of band depletion assay in samples of relapsed/refractory acute leukemia and CML-BC. (A) Densitometry of the 8 AML samples examined by this method before TPT treatment (─ ) and of HL-60 cells subjected to the assay (•, ⋅⋅⋅⋅). To provide a densitometry standard, serial dilutions of control HL-60 cells were included on each blot as depicted in Figs 1 and 2. Truncated lines indicate that the signal was not quantifiable at the next drug concentration. (B) Densitometry of ALL ( ─ ), CML-BC samples (- - -), and K562 CML cells (▪, ⋅⋅⋅⋅) subjected to the same assay. (C) Densitometry of leukemia samples obtained from a single AML patient before and 8 weeks after TPT therapy. Both samples contained greater than 97% blasts. (Inset) TPT accumulation in the paired leukemia specimens before and 8 weeks after TPT therapy. Data are expressed as the mean fluorescence intensity of the clinical specimen divided by the mean fluorescence intensity of KG1a cells subjected to the same assay simultaneously.