Expression of CD40L and CD69 on single positive (SP) T cells and on CD3⁺CD4⁻CD8⁻ (DN) enriched PBMC of patient M.F. The cells were activated with PMA (5 ng/mL) + ionomycin (500 ng/mL) for 16 hours. Dashed lines represent the fluorescence of cells stained with nonimmune mouse IgG. Data are presented as relative log fluorescence intensity.