Peptide 14 blocks the reactivity of G289 MoAbs against E2A/pbx1. Total cell lysate of the 697 line was resolved by SDS-PAGE, transferred to Immobilon-P membrane, and incubated with antibodies G289-781 (lanes 1 and 2), G289-929 (lanes 3 and 4), and G289-1951 (lanes 5 and 6) with (+) or without (−) the peptide 14 added. An E2A-specific antibody G193-86 was used (lanes 7 and 8) as control. Each lane represented the equivalent of 2 × 10⁵ cells. The E2A/pbx1 and E2A signals were detected by enhanced chemiluminescence.