Fig. 6.
VEGF, VRP, and OSM activate the JNK kinase in TrHBMEC. Unstimulated or stimulated TrHBMEC lysates were immunoprecipitated with anti-JNK antibody. The immune complex was incubated with kinase buffer containing 5 μCi γ32P-ATP and 0.2 μg/mL GST-c-Jun at RT for 30 minutes. The reaction products were resolved on 12% SDS-PAGE and detected by autoradiography.
