(A) Association of RAFTK with paxillin in cytokine-treated TrHBMEC. Similar amounts of TCL from TrHBMEC treated with VEGF or bFGF were immunoprecipitated with the anti-RAFTK antibody. The immunoprecipitates were resolved on 7.5% SDS-PAGE, transferred onto a nitrocellulose membrane, and then immunoblotted with anti-paxillin antibody. NRS was used as a negative control. (B) Association of the GST-fusion protein RAFTK C-terminal domain with paxillin. Unstimulated or stimulated TrHBMEC lysates were immunoprecipitated with the GST-fusion protein RAFTK C-terminal domain, separated on 7.5% SDS-PAGE, transferred onto a nitrocellulose membrane, and immunoblotted with the antipaxillin antibody. GST alone was used as a negative control.