Fig. 7.
![Fig. 7. Effects of protease inhibitors on the binding and degradation of 125I-rhTPO by intact platelets. Human PRP was incubated with 125 I-rhTPO with or without cold rhTPO, calpain inhibitor I [Cal (−)], or iodoacetic acid (Iodo) for 60 minutes at 37°C. Binding and degradation of 125I-rhTPO was determined (n = 3 per experiment) as previously described. *Indicates significantly less (P < .05) than total binding (TB), as analyzed by ANOVA followed by a Tukey-Kramer test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2782/4/bl_0002f7.jpeg?Expires=1726836156&Signature=0tOjA2SdXKBR7r-MnJcq7HWN4EKlNwSKN6RPV~iCaxQSKR7ymMrsdYPwNScuM7rION08sN5~04tXZ179hJNpN5l3BpGmzjjcQ8jVrIt5ONUNWloQ7aexH8SWFmCBq2tTvZXDxTe9OLSpd6LcmoX3xGvtrYRfQhF~TZt5UoJ46eFk3O0WOyu3IY-W~xkNV11yB07BYn3gYtGh7xsPv-MIIjwIIXhB2lLS7CDqISFFil9MDJaWFEAUwel7purvA0Q-h0Xw3TCQiGO4r5tVx3THD1uA42SQ7ZQ7LND5akFlcCLGBE5ylbgI6Dki8aGMNLTNVK6dZMIXcBU7UeiZm0Gd2g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of protease inhibitors on the binding and degradation of 125I-rhTPO by intact platelets. Human PRP was incubated with 125 I-rhTPO with or without cold rhTPO, calpain inhibitor I [Cal (−)], or iodoacetic acid (Iodo) for 60 minutes at 37°C. Binding and degradation of 125I-rhTPO was determined (n = 3 per experiment) as previously described. *Indicates significantly less (P < .05) than total binding (TB), as analyzed by ANOVA followed by a Tukey-Kramer test.
