Fig. 5.
Fig. 5. Two-parameter analysis of SHIP expression in lineage-tagged bone marrow cells. Human bone marrow cells were labeled with rat monoclonal antibodies to the various CD markers shown (or a control rat Ig) and affinity-purified antibodies to the murine SHIP protein (or control rabbit Ig). The upper right-hand corner of each graph indicates the combination of antibodies used for cell labeling, with the first representing the FL2 axis and the second the FL1 axis. Thus, SHIP-positive cells appear to the right and CD-positive cells toward the top of each graph. The doubly labeled cells are detectable in the upper right-hand quadrant.

Two-parameter analysis of SHIP expression in lineage-tagged bone marrow cells. Human bone marrow cells were labeled with rat monoclonal antibodies to the various CD markers shown (or a control rat Ig) and affinity-purified antibodies to the murine SHIP protein (or control rabbit Ig). The upper right-hand corner of each graph indicates the combination of antibodies used for cell labeling, with the first representing the FL2 axis and the second the FL1 axis. Thus, SHIP-positive cells appear to the right and CD-positive cells toward the top of each graph. The doubly labeled cells are detectable in the upper right-hand quadrant.

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