Western blot analysis of SHIP protein expression in cells derived from bone marrow or blood. Western blotting with antibodies to murine SHIP was performed on cell lysates derived from different sources. (A) Extracts from murine FDC-P1 cells and murine bone marrow cells (muBM) were compared with extracts from two different human bone marrow (huBM) samples. (B) Peripheral blood leukocytes (PBL) and a CD34⁺ AML sample taken from peripheral blood. (C) Myelomonocytic leukemia cell line, ML-1, either untreated or differentiated to monocytes-macrophages by 2 days of growth in 5 × 10⁻¹⁰ mol/L TPA or to granulocytes by treatment with 1.25% DMSO.