Fig. 6.
Fig. 6. Changes of the cytosolic free calcium concentration [Ca2+]i in HMC-1 cells induced by complement peptides. Fura-2–loaded HMC-1 cells were suspended at 1 × 107 cells/mL and monitored for fluorescence changes after stimulation with different concentrations of C3a (A) and C5a (B). [Ca2+]i changes are expressed as the percentage of total fura-2 saturation. Data represent the mean ± SEM of 3 to 11 experiments for C3a (A, n = 3 to 11) and the mean ± SEM of 4 to 12 experiments for C5a (B, n = 4 to 12).

Changes of the cytosolic free calcium concentration [Ca2+]i in HMC-1 cells induced by complement peptides. Fura-2–loaded HMC-1 cells were suspended at 1 × 107 cells/mL and monitored for fluorescence changes after stimulation with different concentrations of C3a (A) and C5a (B). [Ca2+]i changes are expressed as the percentage of total fura-2 saturation. Data represent the mean ± SEM of 3 to 11 experiments for C3a (A, n = 3 to 11) and the mean ± SEM of 4 to 12 experiments for C5a (B, n = 4 to 12).

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