Adhesion of HMC-1 cells to various extracellular matrix proteins. Wells in 96-well plates were coated with different extracellular matrix proteins at 10 μg/mL. HMC-1 cells were added, and the plates were incubated for 1 hour at 37°C. Plates were gently washed, and the number of adherent cells was determined by measurement of the endogenous hexosaminidase. Adhesion is expressed as the percentage of the total number of cells seeded, corrected for spontaneous adhesion. Data represent the mean ± SD of three experiments performed in triplicate (total n = 9). Spontaneous adhesion to BSA-coated wells was less than 5%.