Flow cytometric analysis of sialic acid-dependent epitope of CD43. Representative results using monoclonal L60 as primary antibody in indirect immunofluorescence of KG1a: Untreated KG1a (histogram curve A); KG1a immediately after neuraminidase treatment (histogram curve B); and KG1a 24 hours after neuraminidase treatment, cultured in the presence of 10 mmol/L sodium chlorate (histogram curve C). Secondary antibody was PE-conjugated. Recovery of sialated surface CD43 is evident despite inhibition of sulfation.