Fig. 2.
Autoradiograph of SDS-PAGE analysis of immunoprecipitated 35S-SO4 metabolically radiolabeled MECA 79 and CD43 proteins from lymph node and KG1a lysates. Immunoprecipitation with MECA 79, anti-CD43, and isotype control MoAbs are as noted above each lane. Numbers at left are MW markers (in kilodaltons). All immunoprecipitates were normalized for equivalent TCA-precipitable counts. A faint band at ∼90 kD is visualized in both IgM control and MECA 79 immunoprecipitates of KG1a lysates. Note the absence of MECA 79-specific protein in KG1a lysates, but prominent CD43 protein signal.
