RP-HPLC chromatograms of 5-lipoxygenase products in enriched monocyte suspensions stimulated with EBV and A23187. Enriched monocyte suspensions (5 × 10⁶ cells/0.1 mL) were preincubated in the absence or in the presence of EBV for 30 minutes at 37°C, diluted 10-fold with HBSS, and further incubated with 75 nmol/L A23187 or DMSO for 5 minutes. (A) Untreated cells; (B) cells stimulated with A23187 only; (C) cells stimulated with EBV only; (D) cells pretreated with EBV and stimulated with A23187; (E) synthetic leukotriene standards. Analyses were performed by RP-HPLC as described in the Materials and Methods. Results shown are from one experiment representative of four separate experiments. The amounts of PGB₂ and 19-OH-PGB₂ (internal standards) in chromatograms (A) through (D) are 12.5 ng.