CpG ODN or IL-2 prestimulation of mononuclear splenocytes in combination with MoAb enhances lysis of 38C13 cells in vitro. Splenocytes were activated as described in the Materials and Methods. Target 38C13 cells were labeled with chromium-51 for 1 hour followed by incubation with MoAb (5 μg/mL) for 30 minutes at 4°C. Effector cells and target cells were plated at a 25:1 effector-target ratio and chromium release was determined.